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Yellow fever mosquitoes (Aedes aegypti) exposed to the insect repellent DEET can learn to associate the chemical with food, according to a study published May 28, 2026, in the Journal of Experimental Biology.
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In the study, researchers housed mosquitoes in a central container connected to two flasks: one containing clean air and the other containing DEET.
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Mosquitoes were allowed to feed on blood from an artificial feeder while exposed to only clean air for 10 seconds, then exposed to DEET in an attempt to train them to associate DEET with a blood meal.
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In a test, trained mosquitoes attempted to bite a hand sprayed with DEET-containing repellent, while untrained mosquitoes avoided it.
Anandasankar Ray, neuroscientist
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Anandasankar Ray, a neuroscientist at the University of California, Riverside, stated, "And [mosquitoes] can be trained to be attracted to it by offering a reward with it."
Anandasankar Ray, neuroscientist
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Anandasankar Ray noted that mosquitoes also smell with their legs and could not land on the DEET-treated hand in the experiments, suggesting that DEET contact would be a punishment rather than a reward.
Clément Vinauger, neuroethologist
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Clément Vinauger, a neuroethologist at Virginia Tech, stated, "There might still be some traces of DEET on the skin, but maybe not enough to create that repellency effect. Mosquitoes might still land, drink some blood and not be repelled."
Clément Vinauger, neuroethologist
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Clément Vinauger stated, "It’s still the gold standard in terms of protection."
Clément Vinauger, neuroethologist
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Clément Vinauger stated, "Flipping that bottle and reading the label is important."
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In another phase of the study, over 60% of mosquitoes returned to a mesh previously associated with blood feeding when only the smell of DEET was reintroduced, even in the absence of blood.
Clément Vinauger, neuroethologist
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Clément Vinauger stated, "Mosquitoes are very smart and can outsmart our control tools. DEET is a molecule that can be interpreted by the mosquito brain, and mosquitoes can learn to like it."
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The study also tested DEET association with sugar feeding and found that mosquitoes avoided sugar treated with DEET unless previously trained to associate DEET with a food reward.
Clément Vinauger, neuroethologist
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Clément Vinauger recommended reapplying DEET at the frequency specified on the product label to maintain effective repellency.
Clément Vinauger, neuroethologist
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Clément Vinauger stated, "It’s the frequency of application, how often you need to put it back on the skin."
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DEET was invented in 1946 by a U.S. Department of Agriculture chemist.
Clément Vinauger, neuroethologist
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Clément Vinauger stated, "Mosquitoes are very plastic, and the more we look at them, the smarter they appear."
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The study was conducted using female yellow fever mosquitoes (Aedes aegypti).
Relevance: supporting · Type: event
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Mosquitoes were tested during their peak blood-seeking hours, early morning and early evening.
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