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Researchers at the University of Arizona R. Ken Coit College of Pharmacy developed a strategy using lipid nanoparticles to enhance immune response against lung cancer tumors.
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The strategy packages the chemotherapy drug paclitaxel and an siRNA molecule in a lipid carrier.
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The findings were published in Nature Communications.
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The lipid package improves delivery of paclitaxel and siRNA to lung tumor cells.
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The approach enhances immunogenic cell death (ICD), a form of cancer cell death that alerts the immune system to tumors.
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Jianqin Lu is the John A. and Frances P. Ware Endowed Associate Professor of Pharmaceutical Sciences in the University of Arizona College of Pharmacy.
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Jianqin Lu is a member of the University of Arizona Comprehensive Cancer Center.
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Jianqin Lu led the research.
Jianqin Lu, Associate Professor of Pharmaceutical Sciences
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"Despite the significant potential of ICD-enabled immunotherapy, its therapeutic applications remain underused."
Jianqin Lu, Associate Professor of Pharmaceutical Sciences
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"Since we know paclitaxel can induce ICD, we improved the delivery of the drug to the tumor by attaching a fatty molecule called a sphingolipid to form a fatty bubble, or nanovesicle."
Jianqin Lu, Associate Professor of Pharmaceutical Sciences
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"We can deliver more drugs to the tumor site."
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When the drug kills cancer cells, the protein calreticulin (CRT) moves to the surface of dying cells.
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Calreticulin acts as a signal that attracts phagocytes, which engulf dying tumor cells and help initiate the ICD response.
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Some tumors use a molecule called STC1 to prevent CRT from reaching the cell surface.
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The prevention of CRT from reaching the cell surface by STC1 weakens the immune response and reduces T cell activity.
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The researchers used an siRNA molecule called siSTC1 to silence STC1 gene activity.
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The siSTC1 was co-delivered with paclitaxel.
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The team tested the strategy in Lewis lung cancer cells, which have high STC1 gene expression.
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The team tested the strategy in MC38 colon cancer cells, which have lower STC1 gene expression.
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The combination of the drug and siSTC1 was more effective against Lewis lung cancer cells than against MC38 colon cancer cells.
Jianqin Lu, Associate Professor of Pharmaceutical Sciences
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"If you trigger an ICD immune response, there should be no tumor development or delayed tumor development."
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In mouse models of lung cancer, the combination of siSTC1 and paclitaxel eradicated three out of five tumors.
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In some cases depending on cancer type, the combination eradicated four out of five tumors in mouse models.
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The siSTC1-paclitaxel package increased tumor sensitivity to PD-1 blockade treatment.
Jianqin Lu, Associate Professor of Pharmaceutical Sciences
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"You're really changing the tumor microenvironment."
Jianqin Lu, Associate Professor of Pharmaceutical Sciences
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"The paclitaxel-siSTC1 combination potentiates the PD-1 blockade therapy, and the cancer immunotherapy."
Jianqin Lu, Associate Professor of Pharmaceutical Sciences
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"That's how this kind of process can have an impact."
Jianqin Lu, Associate Professor of Pharmaceutical Sciences
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Jianqin Lu stated the platform could be used to treat cancer types with high STC1 expression, including non-small cell lung cancer, some colon cancer types, breast cancer, liver cancer, and ovarian cancer.
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The team hopes to collaborate with clinical oncologists and advance the platform in a phase 1 clinical trial.
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Wenpan Li is a researcher/scientist III at the University of Arizona College of Pharmacy and a co-author of the study.
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Zhiren Wang is a former postdoctoral research associate at the University of Arizona College of Pharmacy and a co-author of the study.
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Mengwen Li is a doctoral student in pharmaceutical sciences at the University of Arizona College of Pharmacy and a co-author of the study.
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Yanhao Jiang is a doctoral student in pharmaceutical sciences at the University of Arizona College of Pharmacy and a co-author of the study.
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Shuang Wu is a doctoral student in pharmaceutical sciences at the University of Arizona College of Pharmacy and a co-author of the study.
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Leyla Cordova is a master's degree student in pharmaceutical sciences at the University of Arizona College of Pharmacy and a co-author of the study.
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MinHyeok Kim is a former undergraduate student researcher at the University of Arizona College of Pharmacy and a co-author of the study.
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The journal reference DOI is 10.1038/s41467-026-72526-1.
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